A position is available to support R&D activities at the Gene Transfer Vector Core (GTVC) located at Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye & Ear Infirmary. At the GTVC (https://www.vdb-lab.org/vector-core/), our research and production is focused on developing the scale-up AAV and other viral vectors for supporting research in gene therapies in diseases with unmet need.
This position provides an excellent opportunity to undertake independent responsibilities for diverse research projects in a team context. Selected candidates are required to have relevant experience and be highly motivated to conduct research projects.
1. Molecular cloning of plasmids using PCR, restriction digestion and other methods
2. Downstream protein purification using chromatography, density gradients, and relevant biochemistry methods
3. Cellular assays and in vitro assays to analyze vector properties
4. Process development in AAV and other viral vector production including but not limited to scale-up cell-culture in hyperflasks and small bioreactor. Some training and guidance will be provided.
5. Support Core’s functions in executing projects in timely manner. Maintain diligent records of procedures, databases, and results
6. Performs other laboratory related functions as required. Willingness to train/help others
7. Active Participation in meetings to discuss data, and share/discuss relevant literature
8. Candidate is capable to work independently in experiment design, execution, and data analysis.
EDUCATION AND EXPERIENCE:
• Bachelor’s degree with 5 years’ experience; Masters or PhD in a related field can be substituted for experience
Experience in molecular cloning, PCR
Experience in protein purification, column chromatography etc. biochemistry methods
Experience in cell-culture based functional assays
Cell culturing (bacterial, mammalian cell lines)
• Prior work experience working in a viral vectors or other cores is desirable.
• Experience in planning and designing experiments. Analyzing and interpreting data
• Excellent organization and proactive communication skills for effective teamwork
• Flexibility to take on various projects, multitasking, Creative, problem-solving
• Use of MS Office, and software packages, such as, MS Excel, SnapGene, Geneious. Prism, EndNote)
Work will primarily take place in a research laboratory setting. Job may require exposure to bio-hazardous or toxic substances.
• Position requires alertness and manual dexterity. Possible lifting, maneuvering on cart of 10 to 20 lbs. boxes of generic lab supplies (such as plasticware, solution bottles etc.
HIPAA Privacy Training and Sanctions
All employees who have access to health information whose confidentiality is protected by the HIPAA Privacy Rule are required by the Rule to receive training so that they have an understanding and knowledge of the Privacy Rule that corresponds to their job responsibilities and of the group health plan’s policies and procedures that impact on their job duties. Training on the requirements of the HIPAA Privacy Rule and the plan’s health information policies and procedures under the direction of the institution’s privacy officer is a prerequisite for this position.
Employees who violate the requirements of the HIPAA Privacy Rule will be subject to discipline, up to and including termination.
This description is intended to indicate essential tasks and levels of work difficulty that will be required of positions that will be given this title. It is not intended to limit or in any way modify the right of supervisors to assign, direct, or control the work of staff members under their supervision. The use of a particular expression or illustration describing duties shall not be held to exclude other duties not mentioned but that are of similar kind or level of difficulty.